Cecilia Giulivi

Cecilia Giulivi

Position Title
Professor, Director of the Redox Biology Lab

Department of Molecular Biosciences, School of Veterinary Medicine
MIND Institute

3009 VetMed3B

Profile Introduction

Dr. Giulivi’s expertise is in the area of mitochondria, bioenergetics and free radical biochemistry. This is reflected in >120 publications with more than 7,000 citations in peer-reviewed journals in the chemistry-biochemistry field of mitochondria and free radicals. She has worked on mitochondrial biochemistry since her undergraduate years, and more recently, her focus is on the impact of intermediary metabolism in neurobiology and dietary deficiencies (thiamine), neurodegeneration and neurodevelopmental and neurodegenerative disorders (autism, Huntington’s, fragile X tremor and ataxia syndrome). The effect of gene x environment interaction in autism was explored by using as a model a flame retardant (BDE-49), in which we reported that this additive effect causes a direct disruption of the mitochondrial function at low nM concentrations, further compounded by a genetic background exhibiting a subclinical mitochondrial dysfunction. The study of metabolism in these models is accomplished through the use of a variety of biological models (isolated mitochondria, primary cell culture, established cell cultures, animal models including conditional knock-in and transgenic, human patients’ samples) and using state-of-the art technology (metabolomics, bioinformatics, mitochondrial bioenergetics). Dr. Giulivi has trained a variety of students during the last 20 years, spanning from high-school students (summer internships), undergraduates and graduates to postdoctoral fellows.

Research Interests

Understanding the mitochondrial mechanisms underlying pathophysiology

We explore the mechanisms underlying mitochondrial biology in different pathophysiological cases including autism, schizophrenia, Huntington's and fragile X tremor and ataxia syndrome.

Grad Group Affiliations

  • Biochemistry, Molecular, Cellular and Developmental Biology
  • Forensic Science
  • Integrative Genetics and Genomics

Specialties / Focus

  • Biochemistry
  • Cellular Responses to Toxins and Stress
  • Genomics, Proteomics and Metabolomics
  • Human Genetics and Genomics
  • Molecular Genetics
  • Molecular Medicine
  • Molecular Physiology
  • Neurobiology
  • Organelle and Membrane Biology


  • VetMed VM401 Basic Foundations, Fall
  • VetMed VM405 GI and Metabolism
  • VetMed VM415 Clinical Foundations
  • PMI 201 Integrative Pathobiology Core I, Winter


  • Giulivi's laboratory
    • Eleonora Napoli, Ilaria Marsilio, Nikita Shankar, Sarah Rose, and Thu Q. Nguyen

Honors and Awards

  • 2010 Autism Science Top 10 Achievement for 2010, for groundbreaking work on identifying mitochondrial dysfunction in autistic children.
  • 2011 NIEHS selected as one of the papers of the year (12/2011; DOI: 10.1001/jama.2010.1706).
  • 2012 NIEHS paper of the month (9/2012; PMID: 3416855).

    Professional Societies

    • American Society for Biochemistry and Molecular Biology
    • Society for Free Radical Research


    • 1989 PhD Biochemistry University of Buenos Aires
    • 1985 Diploma Biochemistry University of Buenos Aires


    Giulivi C, Napoli E, Schwartzer J, Careaga M, Ashwood P. Gestational exposure to a viral mimetic poly(i:C) results in long-lasting changes in mitochondrial function by leucocytes in the adult offspring. Mediators Inflamm. 2013;609602. doi: 10.1155/2013/609602. PMID: 24174710; PMCID: 3793312. 

    Napoli E, Hung C, Wong S, Giulivi C. Toxicity of the flame-retardant BDE-49 on brain mitochondria and neuronal progenitor striatal cells enhanced by a PTEN-deficient background. Toxicol Sci. 2013;132(1):196-210. doi: 10.1093/toxsci/kfs339. PMID: 23288049; PMCID: 3693513. 

    Napoli E, Wong S, Giulivi C. Evidence of reactive oxygen species-mediated damage to mitochondrial DNA in children with typical autism. Mol Autism. 2013;4(1):2. doi: 10.1186/2040-2392-4-2. PMID: 23347615; PMCID: 3570390. 

    Napoli E, Ross-Inta C, Wong S, Hung C, Fujisawa Y, Sakaguchi D, Angelastro J, Omanska-Klusek A, Schoenfeld R, Giulivi C. Mitochondrial dysfunction in Pten haplo-insufficient mice with social deficits and repetitive behavior: interplay between Pten and p53. PLoS One. 2012;7(8):e42504. doi: 10.1371/journal.pone.0042504. PMID: 22900024; PMCID: 3416855. 

    Napoli E, Duenas N, Giulivi C. Potential therapeutic use of the ketogenic diet in autism spectrum disorders. Front Pediatr. 2014;2:69. doi: 10.3389/fped.2014.00069. PubMed PMID: 25072037; PMCID: 4074854. 

    Napoli E, Wong S, Hertz-Picciotto I, Giulivi C. Deficits in bioenergetics and impaired immune response in granulocytes from children with autism. Pediatrics. 2014;133(5):e1405-10. doi: 10.1542/peds.2013-1545. PMID: 24753527; PMCID: 4006429. 

    Villanueva C, Giulivi C. Subcellular and cellular locations of nitric oxide synthase isoforms as determinants of health and disease. Free radical biology & medicine. 2010;49(3):307-16. PMCID: 2900489.

    Ross-Inta, C., Tsai, C.-Y., and Giulivi, C. (2008) The mitochondrial pool of free amino acids reflects the composition of mitochondrial-DNA encoded proteins: Indication for a post-translational quality control for protein synthesis. Biosci. Reports 28, 239-49.

    Giulivi, C., Ross-Inta, C.M., Horton, A., and Luckhart, S. (2008) Metabolic Pathways in Anopheles stephensi mitochondria. Biochem. J. 415, 309-16.

    DiMarco, T. and Giulivi, C. (2007) Evaluation of analytical methods to detect dityrosine, a biomarker of oxidative stress, in biological samples. Mass Spec. Rev. 26, 108-120.

    Miller, S., Inta-Ross, C. and Giulivi, C. (2007) Kinetic and proteomic analyses of S-nitrosoglutathione-treated hexokinase: consequences for cancer energy metabolism. Amino acids 32, 593-602.

    Cooper CE and Giulivi, C. (2007) Nitric oxide regulation of mitochondrial oxygen consumption II: molecular mechanism and tissue physiology. Am J Physiol. 292, 1993-2003.

    Giulivi, C., Kato, K. And Cooper, C. E. (2006) Nitric oxide regulation of mitochondrial oxygen consumption I: cellular physiology. Am. J. Physiol. 291, 1225-1231.

    Kato K, Giulivi C. (2006) Critical overview of mitochondrial nitric-oxide synthase. Front Biosci. 11:2725-38.

    Mazzanti, R. and Giulivi, C. (2006) Coordination of nuclear- and mitochondrial-DNA encoded proteins in cancer and normal colon tissues. Biochim Biophys Acta 1757, 618-623.

    Mazzanti, R., Solazzo, M. Squires, R., Fantappie, O., Cechi, F, Bianchi, P., and Giulivi, C. (2006) Differential expression proteomics of human colon cancer. Am J Physiol 290:G1329-38.

    Green, A., Rutherford, M. S., Regal, R. R., Flickinger, G. H., Hendrickson, J. A., Giulivi, C., Mohrman, M. E., Fraser, D. G., and Regal, J. F. (2005) Arginase Activity Differs with Allergen in the Effector Phase of Ovalbumin- versus Trimellitic Anhydride-Induced Asthma. Tox. Sci. 88, 420-433.

    Johnson F, Giulivi C. (2005) Superoxide dismutases and their impact upon human health. Mol Aspects Med. 26, 340-352.

    Oursler, M. J., Bradley, E., Elfering, S. and Giulivi, C. (2005) Native, not nitrated, cytochrome c and mitochondrial-derived hydrogen peroxide drive osteoclast apoptosis. Am., J. Physiol. 288, C156-168.

    Haynes, V., Elfering, S. L., Squires, R., Traaseth, N., Solien, J., Ettl , A. and Giulivi, C. (2004) Mitochondrial Nitric-Oxide Synthase: Role in Pathophysiology. IUBMB Life 55, 599-603.

    Traaseth, N, Elfering, S. L., Solien, J., Haynes, V., and Giulivi, C. (2004) Role of Calcium Signaling in the Activation of Mitochondrial Nitric-Oxide Synthase and Citric Acid Cycle. Biochim. Biophys. Acta 1658, 64-71.

    Haynes, V., Elfering, S. L., Traaseth, N., and Giulivi, C. (2004) Mitochondrial Nitric-Oxide Synthase: Enzyme expression, characterization and regulation. J. Bioenerg. Biomemb. 36, 341-346.

    Elfering, S. L., Haynes, V., Traaseth, N., Ettl, A. and Giulivi, C. (2004) Aspects, mechanism, and biological relevance of mitochondrial protein nitration sustained by mitochondrial nitric oxide synthase. Am. J. Physiol. 286, H22-H29.

    Xiong, S., She, H., Takeuchi, H., Engelhardt, J. F., Barton, C. H., Zandi, E., Giulivi, C., and Tsukamoto, H. (2003) Signaling role of intracellular iron in NF-kB activation. J. Biol. Chem. 278, 17646-54.

    Giulivi, C. (2003) Characterization and function of mitochondrial nitric-oxide synthase. Free Radical Biol. Med. 34, 397-408.

    Giulivi, C, Traaseth, N. J., and Davies, K. J. A. (2003) Tyrosine oxidation products: analysis and biological relevance. Amino Acids 25, 227-232.

    Elfering, S. L., Sarkela, T. M. and Giulivi, C. (2002) Biochemistry of mitochondrial nitric oxide synthase. J. Biol. Chem. 277, 38079-38086.

    Sarkela, T., Berthiaume, J., Elfering, S., Gybina, A. and Giulivi, C. (2001) The modulation of oxygen radical production by endogenous nitric oxide in mitochondria. J. Biol. Chem. 276, 6945-6949.

    Steffen, M., Sarkela, T.M., Gybina, A.A., Steele, T.W., Traaseth, N. J., Kuehl, D. and Giulivi, C. (2001) Metabolism of S-nitrosoglutathione in intact mitochondria. Biochem. J. 356, 395-402.

    French, S., Giulivi, C., and Balaban, R. S. (2001) Nitric oxide synthase in porcine heart mitochondria: Evidence for a low physiological activity. Am. J. Physiol. 280, H2863-7.

    Lizarralde MS, Bailliet G, Poljak S, Fasanella M, Giulivi C. Assessing genetic variation and population structure of invasive North American beaver (Castor Canadensis Kuhl, 1820) in Tierra Del Fuego (Argentina). Biological Invasions. 2008;10(5):673-83.

    Fujisawa Y, Kato K, Giulivi C. Nitration of tyrosine residues 368 and 345 in the beta -subunit elicits F0F1-ATPase activity loss. Biochem J. 2009;423(2):219-31.

    Ross-Inta CM, Zhang YF, Almendares A, Giulivi C. Threonine-deficient diets induced changes in hepatic bioenergetics. American journal of physiology. 2009;296(5):G1130-9. PMCID: 2696218.

    Giulivi C, Zhang Y-F, Omanska-Klusek A, Ross-Inta CM, Wong S, Hertz-Picciotto I, et al. Mitochondrial dysfunction in autism. JAMA. 2010;304:2389-96.

    Haynes V, Traaseth NJ, Elfering S, Fujisawa Y, Giulivi C. Nitration of specific tyrosines in FoF1 ATP synthase and activity loss in aging. Am J Physiol Endocrinol Metab. 2010;298(5):E978-87. PMCID: 2867368.

    Perez CJ, Jaubert J, Guenet J-L, Barnhart KF, Ross-Inta CM, Quintanilla VC, et al. Two Hypomorphic Alleles of Mouse Ass1 as a New Animal Model of Citrullinemia Type I and Other Hyperammonemic Syndromes. American Journal of Pathology. 2010;177(4):1958-68.

    Ross-Inta C, Omanska-Klusek A, Wong S, Barrow C, Garcia-Arocena D, Iwahashi C, et al. Evidence of mitochondrial dysfunction in fragile X-associated tremor/ataxia syndrome. The Biochemical journal. 2010;429(3):545-52.

    Giulivi C, Ross-Inta C, Omanska-Klusek A, Napoli E, Sakaguchi D, Barrientos G, et al. Basal Bioenergetic Abnormalities in Skeletal Muscle from Ryanodine Receptor Malignant Hyperthermia-susceptible R163C Knock-in Mice. Journal of Biological Chemistry. 2011;286(1):99-113.