John A. Payne

John Payne

Position Title

Department of Physiology and Membrane Biology, School of Medicine

4220 Tupper Hall

Profile Introduction

Ion and water homeostasis of excitable cells

Research Interests

Chloride Homeostasis of Excitable Cells

Function and regulation of chloride transporters in neurons and cardiomyoctyes

Molecular Physiology of the Cation Chloride Cotransporters

Molecular function and structure of the chloride transporters, Na-K-Cl cotransporter and K-Cl cotransporter

Grad Group Affiliations

  • Biochemistry, Molecular, Cellular and Developmental Biology
  • Molecular, Cellular and Integrative Physiology
  • Pharmacology and Toxicology

Specialties / Focus

  • Cell Biology
  • Cellular Physiology
  • Comparative Physiology
  • Molecular Physiology
  • Neurophysiology
  • Systemic Physiology


  • HPH 400 Medical Physiology, Summer/Fall
  • HPH 157 Advanced Physiology of Animal/Human Disease, Spring


Honors and Awards

  • Membership, Honor Society of Phi Kappa Phi
  • Established Investigator, American Heart Association

    Professional Societies

    • American Physiological Society
    • Biophysical Society
    • Red Cell Club


    • 1983 BA Biology (marine emphasis) University of California, Berkeley
    • 1987 MS Physiology Duke University
    • 1991 PhD Zoology University of Florida


    Karlócai, M.R., L. Wittner, K. Tóth, Z. Magloczky, Z. Katarova, G. Botond, G. Rásonyi, L. Erőss, S. Czirják, P. Halász, G. Szabó, J.A. Payne, K. Kaila, T.F. Freund.  Enhanced expression of potassium-chloride cotransporter KCC2 in human temporal lobe epilepsy.  Brain Struct. and Funct., 221: 3601-3615, 2016.

    Kaila, K., T.J. Price, J.A. Payne, M. Puskarjov, and J. Voipio.  Cation-chloride cotransporters in neuronal development, plasticity, and disease.  Nature Rev. Neurosci., 15: 637-654, 2014.

    Voipio, J., W.F. Boron, S.W. Jones, U. Hopfer, J.A. Payne, and K. Kaila. Comment on "Local impermeant anions establish the neuronal chloride concentration". Science 345: 1130, 2014.

    Sharp, J.W., C.M. Ross-Inta, I. Baccelli, J.A. Payne, J.B. Rudell, and D.W. Gietzen. Effects of essential amino acid deficiency: downregulation of KCC2 and the GABAA receptor and disinhibition in the anterior piriform cortex. J. Neurochem. 127: 520-530, 2013.

    Antrobus, S.P., C. Lytle, and J.A. Payne. K+-Cl- cotransporter-2 (KCC2) in chicken cardiomyocytes. Am. J. Physiol. Cell Physiol. 303: C1180-C1191, 2012.

    Payne, J.A. Molecular operation of the cation chloride cotransporters: ion binding and inhibitor interaction. Curr. Topics Membr. 70:215-237, 2012.

    Payne, J.A. The potassium-chloride cotransporters: from cloning to structure and function. In "Physiology and Pathology of Chloride Transporters and Channels in the Nervous System: From Molecules to Diseases". (Ed. F.J. Alvarez-Leefmans and E. Delpire). Elsevier, 2009.

    Chung, C.-Y. and J.A. Payne. Modulaton of GABAergic signaling: role of neuronal chloride. In "Encyclopedia of Basic Epilepsy Research", (Ed. P.A. Schwartzkroin). Elsevier, 2009.

    Lee, H.H.C., J.A. Walker, J.R. Williams, R.J. Goodier, J.A. Payne, and S.J. Moss. Direct PKC-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter, KCC2. J. Biol. Chem. 282: 29777-29784, 2007.

    Williams, J.R. and J.A. Payne. Cation transport by the neuronal K-Cl cotransporter, KCC2: Thermodynamics and kinetics of alternate transport modes. Am. J. Physiol. Cell Physiol. 287: C919-C931, 2004.

    Payne, J.A., C. Rivera, J. Voipio, and K. Kaila. Cation chloride cotransporters and their role in neuronal communication, development, and trauma. Trends in Neurosci. 26(4): 199-206, 2003.

    Gulyas, A.I., A. Sik, J.A. Payne, K. Kaila, and T.F. Freund. High expression of the K-Cl cotransporter, KCC2, in postsynaptic membranes associated with excitatory inputs of the rat hippocampus emphasizes a dual role in ion and water homeostasis. Eur. J. Neurosci. 13: 2205-2217, 2001.

    Williams, J.R., J.W. Sharp, V.G. Kumari, M. Wilson, and J.A. Payne. The neuron-specific K-Cl cotransporter, KCC2: antibody development and initial characterization of the protein. J. Biol. Chem. 274(18): 12656-12664, 1999.

    Rivera, C., J. Voipio, J.A. Payne, E. Ruusuvuori, H. Lahtinen, K. Lamsa, U. Pirvola, M. Saarma, K. Kaila. The K+/Cl- co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation. Nature 397: 251-255, 1999.

    Payne, J.A. Functional characterization of the neuronal-specific K-Cl cotransporter: implications for [K+]o regulation. Am. J. Physiol. Cell Physiol.  273: C1516-C1525, 1997.