Manuel F Navedo

Manuel F Navedo

Position Title
Associate Professor

Unit
Department of Pharmacology, School of Medicine

Tupper Hall Room 2419A
Bio

Research Interests

Cellular and molecular mechanisms regulating arterial smooth muscle excitability

The main goal of my research team is to understand the mechanisms by which local and global calcium signals regulate excitation-contraction coupling and excitation-transcription coupling in arterial smooth muscle cells during physiological and pathological conditions. To tackle these issues, we employ several state-of-the-art techniques including molecular biology, patch-clamp electrophysiology, super-resolution microscopy and confocal and Total Internal Reflection Fluorescence (TIRF) microscopy. We are currently interested in determine the mechanisms by which hyperglycemia induces the activation of a novel calcium signaling modality (e.g. persistent calcium sparklets) in arterial smooth muscle, causing increased contraction and consequently, arterial dysfunction during diabetes.

Grad Group Affiliations

  • Molecular, Cellular and Integrative Physiology
  • Pharmacology and Toxicology Graduate Group

Specialties / Focus

  • Cellular Physiology
  • Molecular Physiology
  • Neurophysiology
  • Systemic Physiology

Courses

  • MCP 210C Integrative and comparative physiology, Spring
  • MCP 290 Cellular and Integrative Cardiovascular Biology Journal Club, Fall, Winter, Spring
  • PHA 208 Advanced cardiac physiology and pharmacology, Spring
  • MCP, PTX 215 Electrophysiological and optical techniques to study ion channels, Spring

Labs

  • Navedo Lab
    • Madeline Nieves-Cintron - Assistant Professor In Residence
    • Arsalan U. Syed - Postdoctoral Fellow
    • Debapriya Ghosh - Postdoctoral Fellow
    • Gopireddy R Reddy - Postdoctoral Fellow

Honors and Awards

  • 2007 AHA - Scientist Development Award
  • 2015 National Institutes of Health – Early Career Reviewer Program Alumni
  • 2016-2022 Charter Member NIH VCMB Study Section
  • 2016 UCD Academic Senate Research Travel Award
  • 2019 Fellow of the American Heart Association

    Professional Societies

    • Biophysical Society
    • SACNAS
    • American Physiological Society
    • American Heart Association

    Degrees

    • 2003 PhD Neurobiology University of Puerto Rico
    • 1998 BS General Science University of Puerto Rico

    Publications

    MP Prada, A Syed, OR Buonarati, RG Reddy, MA Nystoriak, D Ghosh, KC Sasse, SM Ward, LF Santana, YK Xiang, JW Hell, M Nieves-Cintron, MF Navedo (2019) A Gs-coupled purinergic receptor boost calcium influx and vascular reactivity during diabetic hyperglycemia. eLife 8: e42214. PMC6397001

    D Ghosh, M Nieves-Cintron, S Tajada, I Brust-Mascher, MC Horne, JW Hell, RE Dixon, LF Santana, MF Navedo (2018) Dynamic L-type CaV1.2 channel trafficking facilitates CaV1.2 clustering and cooperative gating. BBA Molecular Cellular Research 1865: 1341-1355. PMC6407617

    M Nieves-Cintrón, A Syed, O Buonarati, R Rigor, MA Nystoriak, D Ghosh, KC Sasse, SM Ward, LF Santana, JW Hell, MF Navedo (2017) Impaired BKCa channel function in native vascular smooth muscle cells from humans with type 2 diabetes. Scientific Reports 7(1): 14058. PMC5656614

    MA Nystoriak, M Nieves-Cintrón, T Patriarchi, S Morotti, E Grandi, J Dos Santos Fernandes, K Forbush, F Hofmann, KC Sasse, JD Scott, SM Ward, JW Hell, MF Navedo (2017) Ser1928 phosphorylation by PKA stimulates L-type Cav1.2 channels and vasoconstriction during acute hyperglycemia and diabetes. Science Signaling 10, eaaf9647. This article was highlighted in the cover and podcast of the journal and by Faculty of 1000. PMC5297430

    Tags