Manuel F Navedo

Manuel F Navedo

Position Title
Associate Professor

Unit
Department of Pharmacology, School of Medicine

Tupper Hall Room 2419A
Bio

Research Interests

Cellular and molecular mechanisms regulating arterial smooth muscle excitability

The main goal of my research team is to understand the mechanisms by which local and global calcium signals regulate excitation-contraction coupling and excitation-transcription coupling in arterial smooth muscle cells during physiological and pathological conditions. To tackle these issues, we employ several state-of-the-art techniques including molecular biology, patch-clamp electrophysiology, super-resolution microscopy and confocal and Total Internal Reflection Fluorescence (TIRF) microscopy. We are currently interested in determine the mechanisms by which hyperglycemia induces the activation of a novel calcium signaling modality (e.g. persistent calcium sparklets) in arterial smooth muscle, causing increased contraction and consequently, arterial dysfunction during diabetes.

Grad Group Affiliations

  • Molecular, Cellular and Integrative Physiology
  • Pharmacology and Toxicology Graduate Group

Specialties / Focus

  • Cellular Physiology
  • Molecular Physiology
  • Neurophysiology
  • Systemic Physiology

Courses

  • MCP 210C Integrative and comparative physiology, Spring
  • MCP 290 Cellular and Integrative Cardiovascular Biology Journal Club, Fall, Winter, Spring
  • PHA 208 Advanced cardiac physiology and pharmacology, Spring
  • MCP, PTX 215 Electrophysiological and optical techniques to study ion channels, Spring

Labs

  • Navedo Lab
    • Madeline Nieves-Cintron - Research Scientist
    • Maria Paz Prada - Graduate student
    • Arsalan U. Syed - Postdoctoral Fellow
    • Debapriya Ghosh - Postdoctoral Fellow

Honors and Awards

  • 2007 AHA - Scientist Development Award
  • 2015 National Institutes of Health – Early Career Reviewer Program Alumni
  • 2016-2022 Charter Member NIH VCMB Study Section
  • 2016 UCD Academic Senate Research Travel Award

    Professional Societies

    • Biophysical Society
    • SACNAS
    • American Physiological Society
    • American Heart Association

    Degrees

    • 2003 PhD Neurobiology University of Puerto Rico
    • 1998 BS General Science University of Puerto Rico

    Publications

    D Ghosh, M Nieves-Cintron, S Tajada, I Brust-Mascher, MC Horne, JW Hell, RE Dixon, LF Santana, MF Navedo (2018) Dynamic L-type CaV1.2 channel trafficking facilitates CaV1.2 clustering and cooperative gating. BBA Molecular Cellular Research 1865: 1341-1355. PMC in progress

    MA Nystoriak, M Nieves-Cintrón, T Patriarchi, S Morotti, E Grandi, J Dos Santos Fernandes, K Forbush, F Hofmann, KC Sasse, JD Scott, SM Ward, JW Hell, MF Navedo (2017) Ser1928 phosphorylation by PKA stimulates L-type Cav1.2 channels and vasoconstriction during acute hyperglycemia and diabetes. Science Signaling 10, eaaf9647. This article was highlighted in the cover and podcast of the journal and by Faculty of 1000. PMC5297430

    RE Dixon, C Moreno, C Yuan, X Optiz-Araya, M Binder, MF Navedo* LF Santana* (2015) Graded Ca2+/calmodulin-dependent coupling of voltage-gated CaV1.2 channels. eLife 4. *co-corresponding author.

    M Nieves-Cintrón, MA Nystoriak, MP Prada, K Johnson, W Fayer, ML Dell’Acqua, JD Scott, MF Navedo (2015) Selective downregulation of KV2.1 function contributes to enhanced arterial tone during diabetes. JBC 290: 7918-7929.

    MA Nystoriak, M Nieves-Cintrón, PJ Nygren, SA Hinke, CB Nichols, CY Chen, JL Puglisi, L Izu, DM Bers, ML Dell'Acqua, JD Scott, LF Santana, MF Navedo (2014) AKAP150 contributes to enhanced vascular tone by facilitating BKCa channel remodeling in hyperglycemia and diabetes. Circulation Research 114: 607-615. This article is accompanied by an editorial highlighting the novel aspects of the study. PMC3954117.

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