Lecturer; Project Scientist
Department of Molecular and Cellular Biology, College of Biological Sciences
The effect of introns on gene expression.
Introns are often dismissed as junk DNA, but they can have huge effects on gene expression through mechanisms that are not yet understood. I am investigating this interesting phenomenon in plants using molecular genetics, and by testing bioinformatic insights generated by Dr. Ian Korf and his group.
Grad Group Affiliations
- Biochemistry, Molecular, Cellular and Developmental Biology
- Integrative Genetics and Genomics
- Plant Biology
Specialties / Focus
- Gene Regulation
- Model Plants
- Molecular Biology, Biochemistry and Genomics
- Molecular Genetics
- Plant Molecular Biology
- PBI 298 Plant Molecular Biology Discussion
- MCB 120L Biochemistry lab
- BIS 102 Structure and Function of Macromolecules
- MCB 121 Advanced Molecular Biology
- BIS 101 Genes and Gene Expression
- First Year Seminar on Genetically Modified Organisms
- Alan Rose
- 3117 Life Sciences
Honors and Awards
- Award for Excellence in Service to Graduate Students, 2016
- 0 BS Biology University of Waterloo
- 0 MS Molecular Biology York University
- 0 PhD Molecular Biology Princeton University
Gallegos, J.E. and A.B. Rose (2019) An intron-derived motif strongly increases gene expression from transcribed sequences through a splicing independent mechanism in Arabidopsis thaliana. Scientific Reports 9:13777.
Rose, A.B. (2019) Introns as gene regulators: A brick on the accelerator. Frontiers in Genetics 9:672.
Gallegos, J.E. and A.B. Rose (2017) Intron DNA sequences can be more important than the proximal promoter in determining the site of transcript initiation. The Plant Cell 29:843-853.
Rose, A.B., A. Carter, I.F. Korf, and N. Kojima (2016) Intron sequences that stimulate gene expression in Arabidopsis. Plant Molecular Biology 92:337-346.
Gallegos, J. and A.B. Rose (2015) Review: The enduring mystery of intron-mediated enhancement. Plant Science 237:8-15.
Emami, S., D. Arumainayagam, I. Korf, and A.B. Rose (2013) The effects of a stimulating intron on the expression of heterologous genes in Arabidopsis thaliana. Plant Biotechnology Journal, 11:555-563.
Roberts, N.J., G. Morieri, G. Kalsi, A. Rose, J. Stiller, A. Edwards, F. Xie, P.M. Gresshoff, G.E.D. Oldroyd, J.A. Downie, and M.E. Etzler (2013) Rhizobial and mycorrhizal symbioses in Lotus japonicas require lectin nucleotide phosphohydrolase, which acts upstream of calcium signaling. Plant Physiology 161:556-567.
Rose, A.B., S. Emami, K. Bradnam, and I Korf (2011). Evidence for a DNA-based mechanism of intron-mediated enhancement. Frontiers in Plant Science 2:98.
Parra, G., K. Bradnam, A. Rose, and I. Korf (2011). Comparative and functional analysis of intron-mediated enhancement signals reveals conserved features among plants. Nucleic Acids Research 39:5328-5337.
Rose, A.B., T. Elfersi, G. Parra, and I. Korf (2008) Promoter-Proximal Introns in Arabidopsis thaliana are Enriched in Dispersed Signals that Elevate Gene Expression. Plant Cell 20:543-551.
Rose, A.B. (2008). Intron-mediated regulation of gene expression. In Nuclear pre-mRNA Processing in Plants (A.S.N. Reddy and M. Golovkin, eds.) Springer-Verlag, New York. Current Topics in Microbiology and Immunology 326:277-290.
Rose, A.B. (2007) Book Review: Plant Gene Expression. Science STKE, pe26.
Belostotsky, D.A. and A.B. Rose (2005). Plant gene expression in the age of systems biology: Integrating transcriptional and post-transcriptional events. Trends in Plant Science 10:347-353.
Rose, A.B. (2004). The effect of intron location on intron-mediated enhancement of gene expression in Arabidopsis. Plant Journal 40:744-751.
Rose, A.B. (2002). Requirements for intron-mediated enhancement of gene expression in Arabidopsis. RNA. 8:1444-1453.
Shibagaki, N., A. Rose, J.P. McDermott, T. Fujiwara, H. Hayashi, T. Yoneyama, and J.P. Davies (2002). Selenate-resistant mutants of Arabidopsis thaliana identify Sultr1;2, a sulfate transporter required for efficient transport of sulfate into roots. Plant Journal 22:475-486.
Rose, A.B. and J.A. Beliakoff (2000). Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing. Plant Physiology, 122:535-542.
Rose, A.B. and R.L. Last (1997). Introns act post-transcriptionally to increase expression of the Arabidopsis thaliana tryptophan pathway gene PAT1. Plant Journal 11:455-464.
Rose, A.B., J. Li, and R.L. Last (1997). An Allelic Series of Blue Fluorescent trp1 Mutants of Arabidopsis thaliana. Genetics 145:197-205.
Li, J., J. Zhao, A.B. Rose, R. Schmidt, and R.L. Last (1995). Arabidopsis phosphoribosylanthranilate isomerase: Molecular genetic analysis of triplicate tryptophan pathway genes. Plant Cell 7:447-461.
Rose, A.B., A.L. Casselman, and R.L. Last (1992). A phosphoribosylanthranilate transferase gene is defective in blue fluorescent Arabidopsis thaliana tryptophan mutants. Plant Physiology 100:582-592.