J. David D. Furlow

J. David Furlow

Position Title
Professor

Unit
Department of Neurobiology, Physiology and Behavior

274B Briggs Hall
Bio

Profile Introduction

Molecular basis of hormone action, particularly during development

Research Interests

Hormones and Development

Molecular basis of hormone action, particularly during development. Analysis of gene expression programs during morphogenesis. Genome editing in model organisms, particularly applied to nuclear hormone receptor function.

Grad Group Affiliations

  • Biochemistry, Molecular, Cellular and Developmental Biology
  • Molecular, Cellular and Integrative Physiology

Specialties / Focus

  • Cellular Responses to Toxins and Stress
  • Comparative Physiology
  • Developmental Physiology
  • Endocrinology
  • Genomics, Proteomics and Metabolomics
  • Molecular Physiology

Courses

  • NPB 101 Systemic Physiology, Winter
  • NPB 128 Comparative Physiology: Endocrinology, Winter
  • NPB 152 Hormones and Behavior, Spring

Labs

  • Furlow Lab, 274 Briggs Hall
    • Brenda Mengeling

Honors and Awards

  • Whitman Fellow, Marine Biological Laboratory Woods Hole, MA 2014 and 2015
  • Visiting faculty, European Molecular Biology Laboratory, Heidelberg, Germany 2006

    Professional Societies

    • The Endocrine Society
    • Society for Developmental Biology
    • American Physiological Society

    Degrees

    • 1985 BS (Biochemistry) Pennsylvania State University
    • 1992 PhD (Biochemistry) University of Wisconsin, Madison
    • 0 Postdoctoral Fellow - Carnegie Institution of Washington, Department of Embryology

    Publications

    Mengeling BJ, Wei Y, Dobrawa LN, Streekstra M, Louisse J, Singh V, Singh L, Lein PJ, Wulff H, Murk AJ, Furlow JD. A multi-tiered, in vivo, quantitative assay suite for environmental disruptors of thyroid hormone signaling. Aquat Toxicol. 2017 Sep;190:1-10. doi: 10.1016/j.aquatox.2017.06.019. Epub 2017 Jun 21. PubMed PMID: 28662416

    Pérez JH, Furlow JD, Wingfield JC, Ramenofsky M. Regulation of vernal migration in Gambel's white-crowned sparrows: Role of thyroxine and triiodothyronine. Horm Behav. 2016 Aug;84:50-6. doi: 10.1016/j.yhbeh.2016.05.021. Epub 2016 May 24. PubMed PMID: 27234300

    Mengeling BJ, Murk AJ, Furlow JD. Trialkyltin Rexinoid-X Receptor Agonists Selectively Potentiate Thyroid Hormone Induced Programs of Xenopus laevis Metamorphosis. Endocrinology. 2016 Jul;157(7):2712-23. doi: 10.1210/en.2016-1062. Epub 2016 May 11. PubMed PMID: 27167774

    Tandon P, Conlon F, Furlow JD, Horb ME. Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling. Dev Biol. 2017 Jun 15;426(2):325-335. doi: 10.1016/j.ydbio.2016.04.009. Epub 2016 Apr 22. Review. PubMed PMID: 27109192

    Singh L, Pressly B, Mengeling BJ, Fettinger JC, Furlow JD, Lein PJ, Wulff H, Singh V. Chasing the Elusive Benzofuran Impurity of the THR Antagonist NH-3: Synthesis, Isotope Labeling, and Biological Activity. J Org Chem. 2016 Mar 4;81(5):1870-6. doi: 10.1021/acs.joc.5b02665. Epub 2016 Feb 18. PubMed PMID: 26849160.

    Bodine SC, Furlow JD. Glucocorticoids and Skeletal Muscle. Adv Exp Med Biol. 2015;872:145-76.

    Mengeling BJ, Furlow JD. Pituitary specific retinoid-X receptor ligand interactions with thyroid hormone receptor signaling revealed by high throughput  reporter and endogenous gene responses. Toxicol In Vitro. 2015 Oct;29(7):1609-18.

    Freitas J, Miller N, Mengeling BJ, Xia M, Huang R, Houck K, Rietjens IM, Furlow JD, Murk AJ. Identification of thyroid hormone receptor active compounds using a quantitative high-throughput screening platform. Curr Chem Genom Transl Med. 2014 Mar 7;8:36-46

     Furlow JD, Watson ML, Waddell DS, Neff ES, Baehr LM, Ross AP, Bodine SC. Altered gene expression patterns in muscle ring finger 1 null mice during denervation- and dexamethasone-induced muscle atrophy. Physiological Genomics. 2013 Dec 1;45(23):1168-85. 

    Gomes AV, Waddell DS, Siu R, Stein M, Dewey S, Furlow JD, Bodine SC. (2012) Upregulation of proteasome activity in muscle RING finger 1-null mice following denervation. FASEB J. Jul;26(7):2986-99.

    Murk AJ, Rijntjes E, Blaauboer BJ, Clewell R, Crofton KM, Dingemans MM, Furlow JD, Kavlock R, Köhrle J, Opitz R, Traas T, Visser TJ, Xia M, and AC Gutleb (2013) Mechanism-based testing strategy using in vitro approaches for identification of thyroid hormone disrupting chemicals. Toxicology In Vitro. 27(4):1320-1346

    Furlow JD, Watson ML, Waddell DS, Neff ES, Baehr LM, Ross AP, and SC Bodine. (2013) Altered gene expression patterns in muscle ring finger 1 null mice during denervation- and dexamethasone-induced muscle atrophy. Physiological Genomics 45(23):1168-1185.

    Watson ML, Baehr LM, Reichardt HM, Tuckermann JP, Bodine SC, Furlow JD. (2012) A cell-autonomous role for the glucocorticoid receptor in skeletal muscle atrophy induced by systemic glucocorticoid exposure. Am J Physiol Endocrinol Metab. May 1;302(10):E1210-20.

    Files DC, D'Alessio FR, Johnston LF, Kesari P, Aggarwal NR, Garibaldi BT, Mock JR, Simmers JL, DeGorordo A, Murdoch J, Willis MS, Patterson C, Tankersley CG, Messi ML, Liu C, Delbono O, Furlow JD, Bodine SC, Cohn RD, King LS, Crow MT. (2012) A critical role for muscle ring finger-1 in acute lung injury-associated skeletal muscle wasting. Am J Respir Crit Care Med. Apr 15;185(8):825-34.

    Baehr LM, Furlow JD, Bodine SC. (2011) Muscle sparing in muscle RING finger 1 null mice: response to synthetic glucocorticoids. J Physiol. Oct 1;589(Pt 19):4759-76.

    Freitas J, Cano P, Craig-Veit C, Goodson ML, Furlow JD, Murk AJ. (2011) Detection of thyroid hormone receptor disruptors by a novel stable in vitro reporter gene assay. Toxicol In Vitro. Feb;25(1):257-66.

    Denver, RJ, Scanlan TS, and JD Furlow (2009) Thyroid hormone receptor subtype specificity for hormone-dependent neurogenesis in Xenopus laevis. Developmental Biology (in press).

    Johnsen SA, Güngör C, Prenzel T, Riethdorf, S, Riethdorf L, Taniguchi-Ishigaki N, Rau T, Tursun B, Furlow JD, Sauter G, Scheffner M, Pantel K, Gannon F, and I Bach. (2009) Identification of the LIM cofactors CLIM and RLIM as Novel Regulators of Estrogen-Dependent Transcription in Breast Cancer. Cancer Research. 69:128-136

    Waddell DS, Baehr LM, van der Brandt J, Johnsen SA, Reichardt HM, Furlow JD, and SC Bodine (2008) The glucocorticoid receptor and FOXO1 synergistically activate the skeletal muscle atrophy related MuRF1gene. American Journal of Physiology: Endocrinology and Metabolism. 295:E785-797

    Schriks M, Roessig JM, Murk AJ, Furlow JD (2007) Thyroid hormone receptor isoform selectivity of thyroid hormone disrupting compounds quantified with an in vitro reporter gene assay. Environmental Toxicology and Pharmacology 23:320-307.

    Weidner WJ, Waddell DS, Furlow JD. (2006) Measurement of the filtration coefficient (Kfc) in the lung of Gallus domesticus and the effects of increased microvascular permeability. J Comp Physiol [B] 176(6):567-574

    Furlow, J.D. and E.S. Neff (2006) A developmental switch induced by thyroid hormone: Xenopus laevis metamorphosis. Trends in Endocrinolology and Metabolism 17:40-47.

    Schriks M, Zvinavashe E, Furlow JD, Murk AJ. (2006) Disruption of thyroid hormone-mediated Xenopus laevis tadpole tail tip regression by hexabromocyclododecane (HBCD) and 2,2',3,3',4,4',5,5',6-nonabrominated diphenyl ether (BDE206). Chemosphere. 65:1904-1908.

    Lim, W., Neff, E. S., and J. D. Furlow (2004) The mouse muscle creatine kinase promoter faithfully drives reporter gene expression in transgenic Xenopus laevis. Physiological Genomics. 18:79-86

    Furlow J. D., Yang, H. Y., Hsu, Y. M., Lim, W., Ermio, D. J., Chiellini, G., and T. S. Scanlan (2004) The thyroid hormone receptor agonist GC-1 preferentially induces tissue resorption in Xenopus laevis tadpoles. Journal of Biological Chemistry. 279:26555-26562

    Luria, A. and J. D. Furlow (2004) Spatiotemporal activation of retinoid-X receptors detected in live vertebrate embryos. Proceedings of the National Academy of Sciences. 101:8987-8992

    Lim, W, Nguyen, NH, Yang, HY, Scanlan, TS, and JD Furlow (2002) A thyroid hormone antagonist that inhibits thyroid hormone action in vivo. Journal of Biological Chemistry 277:35664-35670

    Lim, W, and JD Furlow (2002) Ribozyme suppression of endogenous thyroid hormone receptor activity in Xenopus laevis cells. Nucleic Acids Research 30:3490-3496

    Furlow, JD and A Kanamori (2002) The Transcription Factor Basic Transcription Element-Binding Protein 1 is a Direct Thyroid Hormone Response Gene in the Frog Xenopus laevis. Endocrinology. 143:3295-3305

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