Roy H. Doi

Roy Doi

Position Title
(In Memoriam) Professor Emeritus

Department of Molecular and Cellular Biology, College of Biological Sciences


Research Interests

Structure, function, and assembly of Clostridium cellulovorans cellulosome (cellulase).

Grad Group Affiliations

  • Microbiology

Field Sites

  • None

Honors and Awards

  • Senior US Scientist Award, Alexander von Humboldt Foundation, 1978-1979
  • Fellow , American Association for the Advancement of Science, 1988.
  • Member, Recombinant DNA Advisory Committee (RAC) of the National Institutes of Health, 1990-1994.
  • Editor, Microbiology and Molecular Biology Reviews, 1998-2006
  • Eminent Scientist of Riken, Riken Institute, Wako, Japan, 1998.
  • Fellow, American Academy of Microbiology, 2001.
  • Distinguished Professor, UC Davis, 2003-present
  • Member, National Academy of Sciences, 2006

    Professional Societies

    • American Society for Microbiology


    • 0 AA 1951 Placer Junior College
    • 0 BA Physiology - 1953 University of California, Berkeley
    • 0 BA Bacteriology - 1957 University of California, Berkeley
    • 0 PhD Bacteriology - 1960 University of Wisconsin, Madison
    • 0 MS Bacteriology - 1958 University of Wisconsin, Madison


    Arai, T., Matsuoka, S., Cho, H.-Y., Yukawa, H., Inui, M., Wong, S.-L., and Doi, R.H. Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation. Proc. Natl. Acad. Sci. USA 104:1456-1460 (2007).

    Arai, T., Kosugi, A., Chan, H., Koukiekolo, R., Yukawa, H., Inui, M. and Doi, R.H. Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans. Appl. Microbiol Biotechnol. 71: 654-660 (2006).

    Kosugi, A., Arai, T. and Doi, R.H. Degradation of cellulosome-produced cello- oligosaccharides by an extracellular non-cellulosomal ²-glucan glucohydrolase, BglA, from Clostridium cellulovorans. Biochem. Biophys. Res. Commun. 349: 20-23 (2006).

    Murashima, K., Kosugi, A., and Doi, R.H. Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans. J. Bacteriol. 187:7146-7149 (2005).

    Han, S.-O., Yukawa, H., Inui, M. and Doi, R.H. Molecular cloning, transcriptional and expression analysis of engO, encoding a non noncellulosomal family 9 enzyme from Clostridium cellulovorans. J. Bacteriol. 187:4884-4889 (2005).

    Doi, R.H. and Kosugi, A. Cellulosomes: Plant Cell Wall Degrading Enzyme Complexes. Nature Reviews - Microbiology 2: 541-551 (2004).

    Cho, H.-Y., Yukawa, H., Inui, M., Doi, R.H. and Wong, S.-L, Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800. Appl. Environ. Microbiol. 70:5704-5707 (2004).

    Kosugi, A., Amano, Y. Murashima, and Doi, R.H. Hydrophjilic domains of scaffolding protein CbpA promote glycosyl hydrolase activity and localization of cellulosomes to the cell surface of Clostridium cellulovorans. J. Bacteriol. 186:6351-6359 (2004).

    Han, S.-O., Cho, H.-Y., Yukawa, H., Inui, M. and Doi, R.H. Regulation of expression of cellulosomes and non-cellulosomal (hemi-)cellulolytic enzymes in Clostridium cellulovorans during growth on different carbon sources. J. Bacteriol. 186:4218-4227 (2004).