Katherine Ralston

Katherine Ralston

Position Title
Assistant Professor

  • Microbiology and Molecular Genetics
266 Briggs Hall
Bio

Research Interests

We focus on interactions between the microbial eukaryote Entamoeba histolytica and the human host. E. histolytica causes amoebiasis, a potentially lethal diarrheal disease in the developing world. The species was named “histolytica” (histo-: tissue; lytic-: dissolving) for its ability to injure host tissue. E. histolytica trophozoites (“amoebae”) possess potent cell-killing activity that is likely to drive tissue damage, but the mechanism by which amoebae kill cells was previously unclear. We discovered that amoebae kill human cells via an unusual mechanism, in which they bite off and ingest human cell fragments that contain human cell membrane, cytoplasm, and organelles. We named this “amoebic trogocytosis” (trogo-: nibble). Trogocytosis provides a new paradigm for the pathogenesis of amoebiasis. As an unusual cell killing insult, it further provides a vehicle to uncover new host cell death and survival signaling.

Unlike phagocytosis (phago-: devour), in which one cell “swallows” another, trogocytosis is poorly understood. However, there is an emerging appreciation that many eukaryotes take bites of membrane from other cells, sometimes together with intracellular contents, suggesting that this process is likely to be fundamental. Trogocytosis plays a role in signaling between immune cells, and intracellular bacteria stimulate host cell trogocytosis to promote their transfer from one cell to another. Other eukaryotic microbes use trogocytosis for cell killing, including the “brain-eating” amoeba, Naegleria fowleri. Therefore, trogocytosis might represent a conserved strategy for eukaryotic intercellular exchange that is exaggerated and exploited for cell killing by microbial pathogens. Our work is therefore broadly relevant to conserved mechanisms for eukaryotic cell-cell interaction.

Education and Degree(s)
  • 2001 B.S. in Genetics, University of California, Davis
  • 2009 Ph.D. in Microbiology, University of California, Los Angeles
Publications
  • Miller, HW, Tam, TSY, and Ralston, KS. (2022). Entamoeba histolytica develops resistance to complement deposition and lysis after acquisition of human complement regulatory proteins through trogocytosis. mBio. 13(2):e0316321.
  • Suleiman, RL, and Ralston, KS. (2022). Growth and genetic manipulation of Entamoeba histolytica. Invited article. Current Protocols. 2(1):e327.
  • Bettadapur, A, Hunter, SS*, Suleiman, RL*, Ruyechan, MC, Huang, W, Barbieri, CG, Miller, HW, Tam, TSY, Settles, ML, and Ralston, KS. (2021). Establishment of quantitative RNAi-based forward genetics in Entamoeba histolytica and identification of genes required for growth. PLOS Pathogens. 17(11):e1010088. *equal authorship 
  • Bettadapur, A, and Ralston, KS. (2020). Direct and high-throughput assays for human cell killing through trogocytosis by Entamoeba histolytica. Molecular and Biochemical Parasitology. 239:111301.
  • Bettadapur, A*, Miller, HW*, and Ralston, KS. (2020). Biting off what can be chewed: Trogocytosis in health, infection and disease. Infection and Immunity. 88:e00930-19. Journal Cover. Part of the Collection: Early-Career Scientists Shaping the New Microbiology. Infection and Immunity. 88:e00129-20. *equal authorship

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